WHOLE AND PAROTID SALIVA – PROTEIN PROFILES AS SEPARATED ON 5-20% SDS-POLYACRYLAMIDE GRADIENT GEL ELECTROPHORESIS AND USING MALDI-TOF MASS SPECTROMETRY
Keywords:
unstimulated whole saliva, stimulated parotid saliva, cerebral palsy, electrophoresis, mass spectrometryAbstract
The aim was to examine the protein profiles of whole and parotid saliva using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and MALDI-TOF mass spectrometry. The banding patterns of proteins exhibited by the unstimulated whole saliva samples on the gel remained quite constant but the intensity of the protein bands were slightly different from one sample to another. Comparison of the protein profiles of unstimulated whole saliva and stimulated parotid saliva showed almost similar banding pattern. The exception is the presence of a pink protein band in the 65-67 kD region in the stimulated parotid saliva samples which was also observed in the unstimulated whole saliva sample contributed by a cerebral palsy patient. Analysis of the saliva samples using MALDI-TOF mass spectrometry also revealed that the stimulated parotid saliva samples exhibited some peaks that were in the same region as those for the unstimulated whole saliva sample of the cerebral palsy subject. This may imply that there is ineffective control of the parotid secretion in cerebral palsy subject under unstimulated condition. The SDS-PAGE and MALDI-TOF analyses may provide more information on the profiles of the salivary proteins which could be beneficial in the diagnosis of salivary gland dysfunction.
Downloads
Downloads
Published
Issue
Section
License
- The Faculty of Dentistry, Universiti Malaya holds the copyright of the published manuscript including all its components such as supplementary information, graphical contents and raw data without any restrictions.
- The author grant the Faculty of Dentistry, University of Malaya assignable and sub-licensable right, unlimited in time and territory, to copy-edit, reproduce, publish, distribute, transmit, make available and store the Article, including abstracts thereof, in all forms of media of expression now known or developed in the future, including pre- and reprints, translations, photographic reproductions and extensions.
- After acceptance for publication in Ann Dent UM, the author(s) have the right to use any of its components in whole or in part for academic purposes such as, presentation in scientific conferences and seminars.