IONIC DISSOLUTION PROFILES OF COMBINED GONIOTHALAMIN AND BIOACTIVE GLASS 45S5 IN CULTURE MEDIUM OF HUMAN OSTEOSARCOMA SAOS-2 CELLS

Received 2022-12-20; Accepted 2023-02-15; Published 2023-06-06

Authors

  • Siti Aishah Abu Bakar Department of Biomedical Science, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia.
  • Nur Asna Azhar Department of Biomedical Science, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia.
  • Noor Muzamil Mohamad Centralised Laboratory Management Center, Universiti Sultan Zainal Abidin, Besut Campus, 22200 Besut, Terengganu Darul Iman, Malaysia.
  • Rokiah Zainuddin Centralised Laboratory Management Center, Universiti Sultan Zainal Abidin, Besut Campus, 22200 Besut, Terengganu Darul Iman, Malaysia.
  • Ira Maya Sophia Nordin Central Research Laboratory, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia.
  • Abdul Manaf Ali Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut Campus, 22200 Besut, Terengganu Darul Iman, Malaysia.
  • Shahrul Bariyah Sahul Hamid Department of Biomedical Science, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia.
  • Siti Noor Fazliah Mohd Noor Department of Dental Science, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia.
  • Nor Hazwani Ahmad Department of Biomedical Science, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Pulau Pinang, Malaysia.

DOI:

https://doi.org/10.22452/jummec.sp2023no1.12

Abstract

This study aimed to investigate the combined effects of goniothalamin and bioactive glass (GTN-BG) on human osteosarcoma Saos-2 cells that emphasise the extracellular changes in the cells' growth medium, including pH and ion release profile. The MTT assay was used to examine the effect of a combination dose of GTN-BG on Saos-2 cells in comparison to untreated and treated Saos-2 with bioactive glass (BG), goniothalamin (GTN), and doxorubicin (DOX). The pH of the culture media for Saos-2 cells was recorded using a pH meter. The release profile of several selected ions such as Ca2+, Na+, Mg2+, K+ and PO43- in the complete culture media of Saos-2 cells was measured by ICP-OES. This study demonstrated that the combination of GTN-BG is more potent than GTN in inhibiting the proliferation of Saos-2 cells while leaving the viability of HMSC as a control normal cell unaffected. The presence of BG significantly increased the pH values in the culture medium of Saos-2. The BG and GTN-BG culture medium exposed to Saos-2 cells showed a significant increment of Ca2+ and Na+ ions concentration after 1 to 24 hours of incubation time compared to UT and GTN. The inhibitory effects of GTN in Saos-2 cells were improved when combined with BG, which may result from a better microenvironment. The release of ionic dissolution products from the BG allowed the rise of pHe, which minimised the pHe/pHi ratio, and subsequently affected the progression of the Saos-2 cells.

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Published

2023-06-06

Issue

Special Issue 1 (2023) Complementary Therapy, Diagnostic and Advanced Material in Medical and Dentistry

Section

Research article

License

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