DEVELOPMENT OF PCR-BASED GENOTYPING METHOD FOR FAT MASS OBESITY-ASSOCIATED (FTO) GENE POLYMORPHISM RS9939609

Received 2023-07-11; Accepted 2023-08-04; Published 2023-09-15

Authors

  • Hartini Yusof Faculty of Health Sciences, UiTM Selangor Branch, Puncak Alam Campus, 42300 Puncak Alam, Selangor.
  • Nur Aimi Mohd Rodzi Faculty of Health Sciences, UiTM Selangor Branch, Puncak Alam Campus, 42300 Puncak Alam, Selangor.
  • Nurhanis Syahira Shakir Faculty of Health Sciences, UiTM Selangor Branch, Puncak Alam Campus, 42300 Puncak Alam, Selangor.
  • Azrina Begam Mohd Ali Apical Scientific Sdn Bhd, No. 7-1 to 7-4, Jln S/P2/7 Taman Serdang Perdana, Seksyen 2 Seri Kembangan, 43300 Seri Kembangan, Selangor, Malaysia.
  • Zana Jamal Kareem Faculty of Health Science, Qaiwan International University (QIU), Slemani Heights, Sulaymaniyah, Iraq.
  • Fazleen Haslinda Mohd Hatta Department of Pharmaceutical Pharmacology and Chemistry, Faculty of Pharmacy, Universiti Teknologi MARA Selangor Branch, Puncak Alam Campus, 42300 Puncak Alam, Selangor.

DOI:

https://doi.org/10.22452/jummec.sp2023no2.5

Abstract

Malaysia has the highest obesity rate in Southeast Asia and is growing. Environmental and genetic factors influence weight increase and obesity, leading to numerous diseases and mortality. This study aimed to develop a genotyping method to detect FTO gene variant rs9939609, known for its association with body mass index (BMI) and obesity. In-house primers detected rs9939609. Two common primers flanked the variation region, and two more primers identified the wild type and alternative allele. Capillary sequencing verified results. We developed FTO gene variant rs9939609 genotyping. This method can be used to genotype FTO variant rs9939609 in any research.

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Published

2023-09-15

Issue

Special Issue 2 (2023): Postgraduate Research Colloquium

Section

Research article

License

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