IN VITRO EVALUATION OF ANTIFUNGAL ACTIVITY OF SELECTED MALAYSIAN PLANTS AGAINST THE WILT PATHOGEN OF BANANA, Fusarium oxysporum f.sp. cubense TROPICAL RACE 4

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Pavitra Paramalingam
Muhammad Salahudin Kheiril Anuar
Nadiya Akmal Baharum
Janna Ong Abdullah
Julia Abd Aziz
Noor Baity Saidi

Abstract

 Fusarium wilt of banana is one of the most serious diseases affecting banana plantations worldwide. In this study, the inhibitory activities of four essential oils (clove, cinnamon, lesser alpinia and eucalyptus) and five hydrosols (kaffir lime, eucalyptus, bitter ginger, fig and tea tree) on the in vitro growth of the causal agent of the disease, Fusarium oxysporum f. sp. cubense Tropical Race 4 (TR4), were investigated. Results showed that some of these plant extracts inhibited the in vitro mycelial growth of TR4 in a dose-dependent manner. Clove oil and tea tree hydrosol were the most effective in suppressing mycelial growth in this study, the with percentage inhibition radial growth (PIRG) recorded at 46% and 69%, respectively, while lesser alpinia oil and kaffir lime hydrosol showed moderate inhibition of mycelial growth with the PIRG of 33% and 64%, respectively. These results suggest that these compounds have the potential to be used in future greenhouse studies as a single treatment or in combination with previously established biological control agents against Fusarium wilt of banana.

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Paramalingam, P. ., Kheiril Anuar, M. S. ., Akmal Baharum, N. ., Abdullah, J. O. ., Abd Aziz, J. ., & Saidi, N. B. (2021). IN VITRO EVALUATION OF ANTIFUNGAL ACTIVITY OF SELECTED MALAYSIAN PLANTS AGAINST THE WILT PATHOGEN OF BANANA, Fusarium oxysporum f.sp. cubense TROPICAL RACE 4. Malaysian Journal of Science, 40(2), 16–24. https://doi.org/10.22452/mjs.vol40no2.2
Section
Original Articles

References

Abd Elsalam K.A. and Khokhlov A.R. (2015) Eugenol oil nanoemulsion:

antifungal activity against Fusarium oxysporum f. sp. vasinfectum and phytotoxicity on cottonseeds. Applied Nanosci 5: 255-265

Abu Bakar, F.I., Abu Bakar, M.F., Abdullah, N., Endrini, S., Rahmat, A. (2018) A review of Malaysian medicinal plants with potential anti-inflammatory activity. Adv Pharmacol Sci 2018: 8603602

Akila R., Rajendran L., Harish S., Saveetha K., Raguchander T., Samiyappan R. (2011) Combined application of botanical formulations and biocontrol agents for the management of Fusarium oxysporum f. sp. cubense (Foc) causing Fusarium wilt in banana. 57: 175-183

Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D.G., Lightfoot, D.A. (2017) Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants. 6: 42.

Bubici G., Kaushal M., Prigigallo M.I., Cabanas C.G-L., Mercado-Blanco J. (2019) Biological control agents against Fusarium wilt of banana. Front Microbiol 10: 616

de Andrade Santiago, J., Cardoso, M.D.G., Batista, L.R., Santiago, W.D., Passamani, F.R.F., Rodrigues, L.M.A., Nelson, D.L. (2018) Effect of the essential oils from Melaleuca alternifolia, Melaleuca quinquenervia and Backhousia citriodora on the synthesis of ochratoxin A by Aspergillus niger and Aspergillus carbonarius isolated from tropical wine grapes. J Food Sci Technol. 55: 418-213.

Dita M., Barquero M., Heck D., Mizubuti E.S.G., Staver C.P. (2018) Fusarium wilt of banana: current knowledge on epidemiology and research needs toward sustainable disease

management. Front Plant Sci 9: 1468DOA (2019) Booklet Statistik Tanaman (Sub-Sektor Tanaman Makanan). Kementerian Pertanian dan Industri Asas Tani, Putrajaya, 48p.

Gnanasekaran P., Salique S.M., Panneerselvam A., Umamagheswari K. (2015) In vitro biological control of Fusarium oxysporum f. sp. cubense by using some Indian medicinal plants. Int J Curr Res Acad Rev 3: 107-116

Huang Y.H., Wang R.C., Li C.H., Zuo C.W., Wei Y.R., Zhang L., Yi G.J. (2012) Control of Fusarium wilt in banana with Chinese leek. Eur J Plant Pathol 134: 87-95

Istianto M. and Emilda D. (2011) Preliminary study of the activity of some essential oils against Fusarium oxysporum f. sp. cubense. J Fruit Ornamental Plant R 19: 111-121

Juniawan, Sastrahidayat I.R., Djauhari S. (2016) Inhibition test of clove leaf oils to Fusarium oxysporum f.sp. cubense. IOSR-JAVS 9: 01-07

Krzyśko-Łupicka, T., Sokół, S., Piekarska-Stachowiak, A. (2020) Evaluation of fungistatic activity of eight selected essential oils on four heterogeneous fusarium isolates obtained from cereal grains in Southern Poland. Molecules. 25: 292

Kumar S., Yadav M., Yadav J.P. (2017) Impact of spatial and climatic conditions on phytochemical diversity and in vitro antioxidant activity of Indian Aloe vera (L.) Burm.f. South African J Bot 111: 50-59

Liu W., Yin D., Li, N., Hou X., Wang D., Li D., Liu J. (2020) Influence of environmental factors on the active substance production and antioxidant activity in Potentilla

fruticosa L. and its quality assessment 6: 28591

Malheiros R.P., de Silva Santos F., Machano L.L., Mapeli A.M. (2019) Phytochemical characterization and effect of Cagaita leaf extracts on Aspergillus Sp. Floresta Ambient 26: e20170029

Mengane S.K. and Kamble S.S. (2014) Bioefficacy of plant extracts on Fusarium oxysporum f.sp. cubense causing panama wilt of banana. Int J Pharm Bio Sci 4: 24-27

Monteiro F.P., Ferreira L.C., Silva J.L., Pacheco L.P., Souza P. E. (2013) Influence of plant extracts and essential oils against panama disease (Fusarium oxysporum f. sp. cubense) in banana seedlings. J Agri Sci 5: 63-74

Olea, A.F., Bravo, A., Martinez, R., Thomas, M., Sedan, C., Espinoza, L., Zambrano, E., Carvajal, D., Silver-Moreno, E., Carasso, H. (2019) Antifungal activity of eugenol derivatives against Botrytis Cinerea. Molecules. 24: 1239

Oliva B., Piccirilli, E., Ceddia, T., Pontieri, E., Aureli, P. andFerrini, A.M. (2003) Antimycotic activity of Melaleuca alternifolia essential oil and its major components. LettAppl Microbiol 37, 185–187

Pegg K.G., Coates L.M., O'Neill W.T., Turner D.W. (2019) The epidemiology of Fusarium wilt of banana. Front Plant Sci 10: 1395

Ploetz R.C. (2015) Fusarium wilt of banana. Phytopathol 105:1512-1521

Šernaitė, L., Rasiukevičiūtė, N., Valiuškaitė, A. (2020) The Extracts of cinnamon and clove as potential biofungicides against strawberry grey mould. Plants(Basel). 9: 613

Terzi V., Morcia C., Faccioli P., Vale G., Tacconi G., Malnati M. (2007) In vitro antifungal activity of the tea tree (Melaleuca alternifolia) essential oil and its major components against plant pathogens. Letters App Microbiol 44: 613-618