THE EFFECTIVENESS OF APPLICATIONS OF BETEL (Piper Betel Linn.) LEAF EXTRACT AND BACTERIOCIN FOR TEAT DIPPING DURING MILKING HANDLING IN INDONESIAN DAIRY FARMING

Main Article Content

Iyep Komala
Irma Isnafia Arief
https://orcid.org/0000-0001-8193-0194
Afton Atabany
Lucia Cyrilla ENSD

Abstract

This study aims to investigate the effectiveness of teat dipping using sirih leaf extract (Piper betle) and bacteria in dairy farms located in Cijeruk Subdistrict, Bogor Regency, West Java. The research employs an experimental design utilizing a Completely Randomized Design (CRD) with four treatments: (1) teat dipping with masofilm (2% iodine) as the positive control, (2) teat dipping with bacteriocin (protein content = 11.17 g mL-1) (P1), (3) teat dipping with betel leaf extract (25% concentration-based) (P2), and (4) teat dipping without any additional treatment, serving as the negative control. Each treatment is replicated three times. The observed parameters include the reduction in somatic cell count (SCC) and total plate count (TPC) in cow's milk. Data were collected through sampling from 12 cows and analyzed using milk quality analysis to assess the conditions of dairy farms in Cijeruk Subdistrict.

Downloads

Download data is not yet available.

Article Details

How to Cite
Komala, I., Arief, I. I., Atabany, A., & ENSD, L. C. (2023). THE EFFECTIVENESS OF APPLICATIONS OF BETEL (Piper Betel Linn.) LEAF EXTRACT AND BACTERIOCIN FOR TEAT DIPPING DURING MILKING HANDLING IN INDONESIAN DAIRY FARMING. Malaysian Journal of Science, 42(2), 7–15. https://doi.org/10.22452/mjs.vol42no2.2
Section
Original Articles

References

Ahmed R N., Bamigboye M O., Ajijolakewu K A., Idris S O., Bamigboye N T A. (2021). Isolation, characterization and antibacterial screening of antibiotics produced from streptomyces isolated from dumpsite soils in Ilorin, North Central Nigeria. Malaysian Journal of Science. 40(3):1-17.

Ajizah A. (2004). Sensitivitas Salmonella typhimurium terhadap ekstrak daun Psidium guajava L. Bioscientiae. 1(1): 31-38.

Aprilia P R., Santoso S A B., Harjanti D W. (2016). Jumlah Staphylococcus aureus dan kandungan nutrien susu akibat dipping puting menggunakan ekstrak daun belimbing wuluh (Averrhoa bilimbi Linn) pada sapi perah penderita mastitis subklinis. Jurnal Ilmu-Ilmu Peternakan. 26(1): 43-51.

Arifin M., Budiman C., Fujiyama K., Arief, I I. (2020). Kinetic and thermodynamic study of plantaricin IIa-1a5, a bacteriocin produced by Indonesian probiotic Lactobacillus plantarum IIa-1a5. Protein and Peptide Letters. 28(6): 680-686.

Arief, I I., Budiman C., Jenie, B S L., Andreas E., Yuneni, A. (2015a). Plantaricin IIA-1A5 from Lactobacillus plantarum IIA-1A5 displays bactericidal activity against Staphylococcus aureus. Beneficial Microbes. 6(4): 603-613.

Arief, I I., Jenie B S L., Astawan M., Fujiyama K., Witarto A B. (2015b). Identification and probiotic characteristics of lactic acid bacteria isolated from Indonesian local beef. Asian Journal Animal Science. 9(1): 25-36.

Arief, I I., Nandika A., Wulandari Z. (2021). Milk quality preservation through the application of plantaricin IIa-1a5 as biopreservatives on fresh milk at dairy cow farm. Agricultural Mechanization in Asia, Africa, and Latin America. 51(3): 1-12.

Bangash M N., Kong M L., Pearse R M. (2012). Use of inotropes and vasopressor agents in critically ill patients. Br J Pharmacol. 165(7): 2015-2033.

Belitz HD., Grosch W., Schieberle P. 2009. Milk and Dairy Products in Food Chemistry. Springer. Berlin.

Caburian, A B., Osi M O. (2010). Characterization and evaluation of antimicrobial activity of the essential oil from the leaves of Piper betle L. E-int. Sci. Res. Journal. 2(2): 1-13.

Fatmarani R., Arief I I., Budiman C. (2018). Purification of bacteriocin from Lactobacillus plantarum IIa-1a5 grown in various whey cheese media under freeze dried condition. Tropical Animal Science Journal. 41(1): 53-59.

Hata E., Katsuda K., Kobayashi H., Uchida I., Tanaka K., Eguchi M. (2010). Genetic variation among Staphylococcus aureus strains from bovine milk and their relevance to methicillin- resistant isolates from humans. Journal of Clinical Microbiology. 48(6): 2130-2139.

Komala I. (2003). Pengaruh ekstrak daun sirih (Piper betle Linn) terhadap bakteri penyebab mastitis. [Skripsi]. Fakultas Peternakan, Institut Pertanian Bogor.

Lubis RR. , Marlisa, Wahyuni DD. 2020. Antibacterial activity of betle leaf (Piper betle l.) extract on inhibiting Staphylococcus aureus in conjunctivitis patient. Am J Clin Exp Immunol 9(1):1-5

Lukman D W., Sudarwanto M., Sanjaya A W., Purnawarman T., Latif H., Soejoedono R R. (2012). Pemeriksaan Mastitis Subklinis. Higiene Pangan Asal Hewan. Bogor: Kesmavet FKH IPB. 35-38.

Muehlhoff E., Bennett A., McMahon D., 2013. Milk and Dairy Products in Human Nutrition. FAO- Rome.

Mutmainna A., Arief I I., Budiman C. (2021). The growth and production of antimicrobial compounds from Lactobacillus plantarum IIA-1A5 on cheese whey medium. J. Indonesian Trop. Anim. Agric. 46(2): 173-184.

Mutaqin B K., Tasripin D S., Adriani L., Tanuwiria U H. (2021). Microbial biomass testing and degree of acidity of milk dairy cows provided complete feed supplementation protein, fat, minerals (pfm) and direct fed microbial. Jurnal Sumber Daya Hewan. 2(1): 1-4.

Noor M A., Apriasari M L. (2014). Antibacterial effectivity of methanol extract from mauli banana stem (Musa acuminata) and 10% povidone iodine against Streptococcus mutans. Jurnal PDGI. 63(2): 12-24.

Pereyra L C., Montana J R G., Benech A., Dibarrat J A., Martin M J., Perini S., Abreu M C., Silva D A., Rodriguez P. (2015). Evaluation of three therapeutic alternatives for the early treatment of ovine pregnancy toxaemia. Irish Veterinary Journal. 68(25): 1-7.

Periyanayagam K., Mubeen M., Basha S S. (2014). Anatomical investigation on the leaves of piper betle (L) var. sirugamani 1 (sgm1) links an ethnomedical important. International Journal of Pharmtech Research. 6(1): 244-251.

Poeloengan M., Komala I., Noor S M., Andriani., Rianti S R P. (2006). Activity water extract of essential oil and ethanol extraction op piper bittle leaves against bacteria isolated from sub clinical mastitis cattle. Seminar Nasional Teknologi Peternakan dan Veteriner.

Priono D., Kusumanti E., Harjanti D W. (2016). Jumlah bakteri Staphylococcus aureus dan skor california mastitis test (cmt) pada susu kambing peranakan etawa akibat dipping ekstrak daun babadotan (Ageratum Conyzoides L.). Jurnal Ilmu-Ilmu Peternakan. 26(1): 52-57.

Riyanto J., Sunarto., Hertanto B S., Cahyadi M., Hidayah R., Sejati W. (2016). Milk quality and production of dairy cow mastitis patients who get treatment of antibiotics. Jurnal Penelitian Ilmu Peternakan. 14(2): 30-41.

Sihombing D E., Arief I I., Budiarti S. (2015). Application of antimicrobial agents produced by Lactobacillus plantarum IIA-1A5 as natural preservative on beef during room temperature storage. Advance Journal Food Sci.Technol. 8(3): 251-255.

Sudono A., Fina., Budi. (2003). Beternak Sapi Perah Secara Intensif. Depok: Penerbit Agromedia Pustaka.

Surjowardojo P. (2011). Incidence level of mastitis with whiteside test on milk yield of dairy cows.

Jurnal Ternak Tropika. 12(1): 46-55.

Steel R G D., Torrie J H., Dicky D A. (1997). Principle and procedure of statistic. A Biometrical Approach. 7.

Tyagi B., Dubey A., Verma AK., Tiwari S. 2015. Antibacterial activity of phenolics compounds against pathogenic bacteria. Int. J. Pharm. Sci. Rev. Res. 35(1) 04: 16-18

Wahyuni S., Kentjonowaty I., Humaidah N. (2021). The effectiveness of herbal dipping teats as a subclinical mastitic prevention. Jurnal Dinamika Rekasatwa. 4(1): 231-249.