The Potential of the Spatholobus littoralis Hassk Plant as an Antioxidant and Prediction of the Mechanism of Activity Against ROS1 Kinase Receptor in Silico

Authors

  • Sri Atun Department Chemistry Education, Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta, Jl. Colombo No.1 Depok, Sleman, Yogyakarta, 55281, INDONESIA. https://orcid.org/0000-0002-6225-4255
  • Nurfina Aznam Department Chemistry Education, Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta, Jl. Colombo No.1 Depok, Sleman, Yogyakarta, 55281, INDONESIA. https://orcid.org/0000-0002-0818-0136
  • Rasningtyaswati Department Chemistry Education, Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta, Jl. Colombo No.1 Depok, Sleman, Yogyakarta, 55281, INDONESIA. https://orcid.org/0009-0003-1880-076X
  • Putri Verdiana Dwi Cahyani Department Chemistry Education, Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta, Jl. Colombo No.1 Depok, Sleman, Yogyakarta, 55281, INDONESIA. https://orcid.org/0009-0001-2223-5623
  • Lusiana Qotimatul Izah Department Chemistry Education, Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta, Jl. Colombo No.1 Depok, Sleman, Yogyakarta, 55281, INDONESIA. https://orcid.org/0009-0004-5472-6242
  • Wiwid Deswantari Danarjati Department Chemistry Education, Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta, Jl. Colombo No.1 Depok, Sleman, Yogyakarta, 55281, INDONESIA. https://orcid.org/0009-0002-7947-6759
  • Adity Sangal Sangal Department of Chemistry, Amity Institute of Applied Sciences, Amity University, Noida (U.P.) INDIA. https://orcid.org/0000-0001-5996-1680

DOI:

https://doi.org/10.22452/mjs.vol44no2.4

Keywords:

Spatholobus littoralis Hassk, antioxidant, ROS1 kinase receptors, In silico

Abstract

Various phenolic compounds that exhibit antioxidant, anticancer, and anti-inflammatory activities are found in Spatholobus. Therefore, this study aimed to determine the antioxidant potential of Spatholobus littoralis wood in vitro and predict the mechanism of its activity against the ROS1 kinase receptor in silico. The ground-dried wood of S. littoralis was extracted with ethanol via maceration. The analysis of the total phenolic content (TPC) of extracts and fractions obtained from S. littoralis wood was determined by the Folin-Ciocalteu reagent. Similarly, the antioxidant activity was determined by the DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (The ferric reducing antioxidant power) method. The human ROS1 kinase enzyme (4UXL and 3ZBF) was used to determine the molecular mechanism of the interaction from the genus Spatholobus in silico. The total ethanol extract, chloroform, and ethyl acetate fraction of S. littoralis showed a high content of phenolic compounds and antioxidant activity. Phenolic compounds in plants of the genus Spatholubus also showed good activity against ROS1 kinase receptors (3ZBF and 4UXL). In conclusion, the S. littoralis plant has the potential to be developed for the discovery of new drugs.

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Published

30-06-2025

How to Cite

Atun, S., Aznam, N., Rasningtyaswati, Cahyani, P. V. D. ., Izah, L. Q. ., Danarjati, W. D. ., & Sangal, A. S. (2025). The Potential of the Spatholobus littoralis Hassk Plant as an Antioxidant and Prediction of the Mechanism of Activity Against ROS1 Kinase Receptor in Silico. Malaysian Journal of Science (MJS), 44(2), 42–51. https://doi.org/10.22452/mjs.vol44no2.4

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Vol 44 No 2 (2025): June 2025

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