Guanidine hydrochloride-induced denaturation of bovine serum albumin: a comparative study and analysis using different probes

Article Sidebar

PDF
Published: Aug 28, 2008

Main Article Content

Adyani Azizah Abd. Halim
Habsah Abdul Kadir
Tayyab Saad

Abstract

The denaturation of bovine serum albumin (BSA) by guanidine hydrochloride (GdnHC1) showed a single-step, two-state transition, when monitored by different probes such as intrinsic fluorescence at 338 and 333 nm after excitation at 280 and 295 nm respectively, UV difference spectral signal at 288 nm, 1-anilinonaphthalene-8-sulphonate (ANS) fluorescence at 470 nm after excitation at 380 nm, bromophenol blue (BPB)-induced difference spectral signal at 619 nm and A.,„„ of positive difference spectral signal of BPB-BSA complex. A comparison of the denaturation curves obtained with the above mentioned probes showed differences in the requirement of GdnHC1 concentration• for the transition to start and complete. The values for the mid-point of denaturation transition and free energy change associated with GdnHC1 denaturation (AG Du2o.) also varied from each other, using different probes.

Downloads

Download data is not yet available.

Article Details

How to Cite
Abd. Halim, A. A., Abdul Kadir, H., & Saad, T. (2008). Guanidine hydrochloride-induced denaturation of bovine serum albumin: a comparative study and analysis using different probes. Malaysian Journal of Science, 27(2), 9–12. Retrieved from https://ejournal.um.edu.my/index.php/MJS/article/view/7886
Issue
Vol. 27 No. 2 (2008)
Section
Original Articles

Transfer of Copyrights

  •  In the event of publication of the manuscript entitled [INSERT MANUSCRIPT TITLE AND REF NO.] in the Malaysian Journal of Science, I hereby transfer copyrights of the manuscript title, abstract and contents to the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) for the full legal term of copyright and any renewals thereof throughout the world in any format, and any media for communication.

 

Conditions of Publication

  •  I hereby state that this manuscript to be published is an original work, unpublished in any form prior and I have obtained the necessary permission for the reproduction (or am the owner) of any images, illustrations, tables, charts, figures, maps, photographs and other visual materials of whom the copyrights is owned by a third party.
  • This manuscript contains no statements that are contradictory to the relevant local and international laws or that infringes on the rights of others.
  • I agree to indemnify the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) in the event of any claims that arise in regards to the above conditions and assume full liability on the published manuscript.

 

Reviewer’s Responsibilities

  • Reviewers must treat the manuscripts received for reviewing process as confidential. It must not be shown or discussed with others without the authorization from the editor of MJS.
  • Reviewers assigned must not have conflicts of interest with respect to the original work, the authors of the article or the research funding.
  • Reviewers should judge or evaluate the manuscripts objective as possible. The feedback from the reviewers should be express clearly with supporting arguments.
  • If the assigned reviewer considers themselves not able to complete the review of the manuscript, they must communicate with the editor, so that the manuscript could be sent to another suitable reviewer.

 

Copyright: Rights of the Author(s)

  • Effective 2007, it will become the policy of the Malaysian Journal of Science (published by the Faculty of Science, University of Malaya) to obtain copyrights of all manuscripts published. This is to facilitate:
  • Protection against copyright infringement of the manuscript through copyright breaches or piracy.
  • Timely handling of reproduction requests from authorized third parties that are addressed directly to the Faculty of Science, University of Malaya.
  • As the author, you may publish the fore-mentioned manuscript, whole or any part thereof, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given. You may produce copies of your manuscript, whole or any part thereof, for teaching purposes or to be provided, on individual basis, to fellow researchers.
  • You may include the fore-mentioned manuscript, whole or any part thereof, electronically on a secure network at your affiliated institution, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
  • You may include the fore-mentioned manuscript, whole or any part thereof, on the World Wide Web, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
  • In the event that your manuscript, whole or any part thereof, has been requested to be reproduced, for any purpose or in any form approved by the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers), you will be informed. It is requested that any changes to your contact details (especially e-mail addresses) are made known.

 

 Copyright: Role and responsibility of the Author(s)

  • In the event of the manuscript to be published in the Malaysian Journal of Science contains materials copyrighted to others prior, it is the responsibility of current author(s) to obtain written permission from the copyright owner or owners.
  • This written permission should be submitted with the proof-copy of the manuscript to be published in the Malaysian Journal of Science